cd62l fitc mel-14 antibody  (Thermo Fisher)

Journal: Cells

Article Title: Endoplasmic Reticulum Protein Disulfide Isomerase Shapes T Cell Efficacy for Adoptive Cellular Therapy of Tumors

doi: 10.3390/cells8121514

Figure Lengend Snippet: PDI inhibition promotes traits associated with T cell tumor control. Pmel T cells were activated with gp100 peptide and expanded in the presence of vehicle, IL-15, or E64FC26. ( A ) Representative FACS plots and quantification of CD62L and CD44 expression and ( B – D ) RT-PCR was used to measure expression of indicated genes. Data points represent average values from three separate experiments. Significance represents vehicle versus E64FC26 values, IL-15-primed T cells values are shown as a reference point. ( E ) Oxygen consumption rate (OCR) trace with injection markers and ( F – H ) quantification of indicated values from Seahorse Bioanalysis in response to mitochondrial stress test. Individual experiment performed three times. Differences were considered significant when * p < 0.05, ** p < 0.01, *** p <0.001, **** p < 0.0001.

Article Snippet: Fluorochrome-conjugated monoclonal antibodies CD62L-FITC (MEL-14), CD44-PE-Cy7 (IM7), and CD8-APC (53-6.7) and respective isotype controls were purchased from Thermo Fisher (Waltham, MA, USA).

Techniques: Inhibition, Control, Expressing, Reverse Transcription Polymerase Chain Reaction, Injection